Insertion junction: LMJ.RY0402.167819_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.167819
Chromosome:chromosome 12
Location:7517827
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g555950 DHC11 Axonemal Dynein Heavy Chain 11; (1 of 1) PTHR10676//PTHR10676:SF265 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGTTGTTGCAAAGACTGATGAGGGAAGC
Internal bar code:AGTAATGTTGGTCTTTGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:937
LEAP-Seq percent confirming:99.3939
LEAP-Seq n confirming:328
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTTCGTGGTCAGGTACG
Suggested primer 2:ACAGTGTCACAAGGAAGGGC