| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167830 |
| Chromosome: | chromosome 2 |
| Location: | 6278840 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114500 | (1 of 1) 3.1.3.75 - Phosphoethanolamine/phosphocholine phosphatase / PHOSPHO1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCATAACCCCGTGGTCCTGACGTGCGC |
| Internal bar code: | TATGATATGGTAATTCGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 99.7842 |
| LEAP-Seq n confirming: | 2312 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGGAACTCAGTCAGGC |
| Suggested primer 2: | TTCCTACCAACGCTACCCAC |