| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167831 |
| Chromosome: | chromosome 6 |
| Location: | 8735515 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309800 | MOT57,MOT2 | Predicted protein in motile organisms; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCATTCAGCTGCTAGGGGGCTCCGCATT |
| Internal bar code: | CCCTGTAGTTTACGAAGAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1263 |
| LEAP-Seq percent confirming: | 99.0 |
| LEAP-Seq n confirming: | 297 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCGCTATCTAGCTTTGG |
| Suggested primer 2: | AAATGCATGCAGTCAAGCTG |