| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167831 |
| Chromosome: | chromosome 6 |
| Location: | 8735516 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309800 | MOT57,MOT2 | Predicted protein in motile organisms; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATGCCCATGCCCCCGTTCTGTAAGATGT |
| Internal bar code: | GAATCTGAGGGGCTTTCGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 99.8284 |
| LEAP-Seq n confirming: | 1745 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCGCTATCTAGCTTTGG |
| Suggested primer 2: | AAATGCATGCAGTCAAGCTG |