Insertion junction: LMJ.RY0402.167840_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.167840
Chromosome:chromosome 7
Location:1618699
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325050 ELG15 Exostosin-like glycosyltransferase 15; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGGTAACAAATGGTGGATGTATCTTCA
Internal bar code:AGTGACAATCAAAGGAATGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:262
LEAP-Seq percent confirming:99.6357
LEAP-Seq n confirming:1094
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATGTGCCTTCCCTGAAC
Suggested primer 2:GACAACAGGACGATGTGTGG