Insertion junction: LMJ.RY0402.167840_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.167840
Chromosome:chromosome 7
Location:1618965
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325050 ELG15 Exostosin-like glycosyltransferase 15; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTGCACTAGGTCAAGACGAGGAACGAA
Internal bar code:TTGTACATGGTGTTTGGCGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:255
LEAP-Seq percent confirming:96.8646
LEAP-Seq n confirming:1452
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGATCTGCGGTCAGCAC
Suggested primer 2:CTGTTCACACACGATGAGGG