| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167859 |
| Chromosome: | chromosome_17 |
| Location: | 1210233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g705000 | CDPK1 | Calcium-dependent protein kinase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCATTGGCACGTGGGTGGGTGGCGTGCATG |
| Internal bar code: | GCGGAAACCGGGAGGACGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1149 |
| LEAP-Seq percent confirming: | 99.7502 |
| LEAP-Seq n confirming: | 1597 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTACAATCCACCAGGCGT |
| Suggested primer 2: | AACATCTACACCGGCCTCAC |