| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167859 |
| Chromosome: | chromosome 17 |
| Location: | 1210249 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705000 | FAP223,CDPK1 | (1 of 1) PF00069//PF00168//PF13499//PF13833 - Protein kinase domain (Pkinase) // C2 domain (C2) // EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8); Flagellar Associated Protein 223 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCTGTCCATCGCCTCGTGTCGCGTTCTT |
| Internal bar code: | CTGTTTACCGTGTATAGAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 99.333 |
| LEAP-Seq n confirming: | 3872 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTACAATCCACCAGGCGT |
| Suggested primer 2: | AACATCTACACCGGCCTCAC |