| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167869 |
| Chromosome: | chromosome 13 |
| Location: | 1638367 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573700 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTGGAGGCGAGTCTGGCGGCGCGCGTG |
| Internal bar code: | GTCTCACATGCCTTATAAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 99.1548 |
| LEAP-Seq n confirming: | 4927 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCAGTTGACATGGTATTG |
| Suggested primer 2: | GAGTTGACGCTGACGTGTGT |