| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167871 |
| Chromosome: | chromosome 2 |
| Location: | 5997663 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112250 | CAL2 | (1 of 1) 3.4.22.53//3.4.22.54 - Calpain-2 / Milli-calpain // Calpain-3 / Muscle-specific calcium-activated neutral protease 3; Calpain family cysteine protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGTTGCCAGGCGGTTTGGGGTTTCCCA |
| Internal bar code: | GCGCTCCCCGAGTTGGAAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 904 |
| LEAP-Seq percent confirming: | 99.5855 |
| LEAP-Seq n confirming: | 961 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCGTCTGCTTATCTTAG |
| Suggested primer 2: | GTGGTAGATGTGCGTGATGG |