Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.167892 |
Chromosome: | chromosome 1 |
Location: | 5554209 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g039450 | (1 of 2) PTHR34548//PTHR34548:SF2 - FAMILY NOT NAMED // PROTEIN TIC 21, CHLOROPLASTIC | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGACCCCGCGCGCGTGTGCGGCCCGCTT |
Internal bar code: | CTCGTTCTCGGCCTATATGTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 41 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 22 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTACCGCATCATACGCACA |
Suggested primer 2: | TCGTATGCTTCTGTTGACGC |