| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167901 |
| Chromosome: | chromosome 12 |
| Location: | 4709105 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523550 | UMM11 | UbiE/COQ5 methyltransferase family protein; (1 of 1) PTHR10108//PTHR10108:SF752 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGATTTGAGACTCGGTCACCCATGCGG |
| Internal bar code: | CCCGGCTTGGGTCGCTGGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1010 |
| LEAP-Seq percent confirming: | 97.9967 |
| LEAP-Seq n confirming: | 587 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTTCACTCTGCTCCTCC |
| Suggested primer 2: | AAGTTCCCATCATGTCTGCC |