| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167908 |
| Chromosome: | chromosome 12 |
| Location: | 1013535 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491500 | UBA2,UBA3,UBC1 | RUB1/NEDD8 E1 activating enzyme; (1 of 1) K10686 - ubiquitin-activating enzyme E1 C (UBE1C, UBA3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCAGATGATAGTTGGTATAAGTTGGTT |
| Internal bar code: | GATGGTAGGGAGGGTAGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 99.8908 |
| LEAP-Seq n confirming: | 915 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACACCCACTTCATGTGC |
| Suggested primer 2: | GGAGATTGGGATGGGTTACA |