Insertion junction: LMJ.RY0402.167911_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.167911
Chromosome:chromosome 6
Location:3567262
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278118 5'UTR_intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATGCGTTGGGGCCGCGGGTGGATTGTAA
Internal bar code:CCGGAGACCGAAATGCGAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:421
LEAP-Seq percent confirming:18.5348
LEAP-Seq n confirming:1265
LEAP-Seq n nonconfirming:5560
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAGCACAACAGGTGAGCA
Suggested primer 2:CAGGTAGGTAGCTTGGCGAG