| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167939 |
| Chromosome: | chromosome_5 |
| Location: | 3212866 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g239850 | PHO4,PHOD | Alkaline phosphatase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTGGGCCAGCGTGCTGAAGAAGGCCAAC |
| Internal bar code: | AAAGCATGGTCATGACCGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.5921 |
| LEAP-Seq n confirming: | 25883 |
| LEAP-Seq n nonconfirming: | 106 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTGTGAGATAGGTGCGA |
| Suggested primer 2: | CACCTCACACAATCACCCTG |