| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167953 |
| Chromosome: | chromosome 16 |
| Location: | 1981076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656551 | TRL14,TRXL14,PDI | (1 of 10) 5.3.4.1 - Protein disulfide-isomerase / S-S rearrangase; Thioredoxin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAATCAGCCGACATATGCATTCCATTT |
| Internal bar code: | CTTTCTGTGTACCAGCCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGCTTTCTGTTTTCAGC |
| Suggested primer 2: | CAGCTTGCTACAGGTCCTCC |