| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167953 |
| Chromosome: | chromosome 16 |
| Location: | 1981082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656551 | TRL14,TRXL14,PDI | (1 of 10) 5.3.4.1 - Protein disulfide-isomerase / S-S rearrangase; Thioredoxin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGTTGGGGGTTCCCAGGCCACATGCA |
| Internal bar code: | TACGAGGCCCGTTAGGCTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 99.8016 |
| LEAP-Seq n confirming: | 1509 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGCTTTCTGTTTTCAGC |
| Suggested primer 2: | CAGCTTGCTACAGGTCCTCC |