| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167958 |
| Chromosome: | chromosome 2 |
| Location: | 844999 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078950 | MMP17 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGGAGGATCAAGCATGTTGACATTAG |
| Internal bar code: | AGGATGCACGCAACGAAACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 98.0831 |
| LEAP-Seq n confirming: | 147825 |
| LEAP-Seq n nonconfirming: | 2889 |
| LEAP-Seq n unique pos: | 274 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCGGGTTATTCGTGTGCT |
| Suggested primer 2: | GACGCAACTAACAATCCGGT |