Insertion junction: LMJ.RY0402.167958_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.167958
Chromosome:chromosome 2
Location:844999
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g078950 MMP17 Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTTGGAGGATCAAGCATGTTGACATTAG
Internal bar code:AGGATGCACGCAACGAAACGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:820
LEAP-Seq percent confirming:98.0831
LEAP-Seq n confirming:147825
LEAP-Seq n nonconfirming:2889
LEAP-Seq n unique pos:274

Suggested primers for confirmation by PCR

Suggested primer 1:GATCGGGTTATTCGTGTGCT
Suggested primer 2:GACGCAACTAACAATCCGGT