| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167967 |
| Chromosome: | chromosome 10 |
| Location: | 4482607 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451752 | PHC25 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCTATCTATTAAACCTACAAAGCCCA |
| Internal bar code: | CATTGACCGCGAGTTCGGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 98.8176 |
| LEAP-Seq n confirming: | 3510 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCAGGTTACACAAGGGT |
| Suggested primer 2: | ACACACACACACACACACCG |