| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168001 |
| Chromosome: | chromosome 3 |
| Location: | 4097085 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172900 | (1 of 2) PF11371 - Protein of unknown function (DUF3172) (DUF3172) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGAGAGAAGAGACCGTGTTTCCCGTGA |
| Internal bar code: | CGGGAGAGTACTGAAGACAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.7632 |
| LEAP-Seq n confirming: | 5476 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTAGTGTGCACGGAACC |
| Suggested primer 2: | TCATGCGCTAACAGGCTATG |