| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168055 |
| Chromosome: | chromosome 8 |
| Location: | 2640240 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373050 | BCC3,GEX104 | (1 of 1) K11262 - acetyl-CoA carboxylase / biotin carboxylase (ACAC); Acetyl-CoA biotin carboxyl carrier | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGAATCGGGCGCCTTGCTTCCAGATGG |
| Internal bar code: | TTGATACATTAGTATTGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 99.09 |
| LEAP-Seq n confirming: | 7187 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGCTTACCATCCACGTT |
| Suggested primer 2: | GGCTTGGGCTGGTATTTACA |