| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168076 |
| Chromosome: | chromosome 7 |
| Location: | 3644160 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGTGCCCCTCTGCCTTGCAGTATTTCG |
| Internal bar code: | GCCGACGGGTGTTTTACTTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 25.6061 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 491 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGGTGAGTTCCTGATGT |
| Suggested primer 2: | GAACAAGCTTCGCAAAGGAC |