| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168089 |
| Chromosome: | chromosome 2 |
| Location: | 5961562 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112000 | AOT7 | Amino acid transporter; (1 of 1) K14997 - solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 11 (SLC38A11) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGCTCCGGTCTGATGTGCTCATCGCT |
| Internal bar code: | TCCACCGCGCTGCACGTGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 253 |
| LEAP-Seq percent confirming: | 33.2724 |
| LEAP-Seq n confirming: | 2547 |
| LEAP-Seq n nonconfirming: | 5108 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGCGAGGGACAGAAAG |
| Suggested primer 2: | CATGGGATGCATGTAGAACG |