| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168090 |
| Chromosome: | chromosome 9 |
| Location: | 6771656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409350 | RLI1 | (1 of 1) K06174 - ATP-binding cassette, sub-family E, member 1 (ABCE1); Fe-S ABC transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTAGATACAGAAGACGCTAATCGTGGG |
| Internal bar code: | CCGCAATTTTTATCTCACATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1243 |
| LEAP-Seq percent confirming: | 98.6069 |
| LEAP-Seq n confirming: | 9414 |
| LEAP-Seq n nonconfirming: | 133 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACTTGGCGGTCATACCT |
| Suggested primer 2: | ACCCGTACTCCTCCACTCCT |