Insertion junction: LMJ.RY0402.168123_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.168123
Chromosome:chromosome 16
Location:2674031
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g662100 (1 of 10) IPR004087//IPR004088 - K Homology domain // K Homology domain, type 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCGCGAGCTTAGCAAGGGCGCCGAGAG
Internal bar code:CAAGAGGCGGGTCGTGCGGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:781
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:150
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTACGGGTAGTCACGGGT
Suggested primer 2:TTGAGCGAGTGGAGGAGAAT