| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168140 |
| Chromosome: | chromosome 10 |
| Location: | 4543188 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452250 | FAP41 | Flagellar Associated Protein 41 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGAACAATAGGAATACCCCCTGGTGCA |
| Internal bar code: | ACTGGTAGGGTGCGACGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 99.6764 |
| LEAP-Seq n confirming: | 1848 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAACTTTGTCGATCAGC |
| Suggested primer 2: | CCGTTGTGTTCGTGTCAATC |