| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168144 |
| Chromosome: | chromosome 12 |
| Location: | 8150888 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551201 | NAT28 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTCTATTGAGCTACCATCACCCCAAAA |
| Internal bar code: | GCGCGGGGCGTCTGGTAACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1199 |
| LEAP-Seq percent confirming: | 97.8359 |
| LEAP-Seq n confirming: | 1944 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCACGTGGTTGGGAGTT |
| Suggested primer 2: | CGGTCTTAGCACTTTCTGCC |