| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168159 |
| Chromosome: | chromosome_17 |
| Location: | 4321878 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g730950 | KIN2-2,KHP1,FLA10 | Kinesin motor protein, kinesin-2 (KRP85/95) family | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGATCGGCATGCATGCTAGCAACCTGCGC |
| Internal bar code: | TGACGCCCTTATTACTCTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 98.4061 |
| LEAP-Seq n confirming: | 2593 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTATGTACGGCCATCAG |
| Suggested primer 2: | GGAAACGCGACACATACCTT |