Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.168159 |
Chromosome: | chromosome_17 |
Location: | 4321919 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g730950 | KIN2-2,KHP1,FLA10 | Kinesin motor protein, kinesin-2 (KRP85/95) family | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGGTACTCACTTGCCGCCTGTGCGCCGCGC |
Internal bar code: | GACGCCGCCCAGGAGTCCGTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 416 |
LEAP-Seq percent confirming: | 96.1538 |
LEAP-Seq n confirming: | 325 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACGTATGTACGGCCATCAG |
Suggested primer 2: | GGAAACGCGACACATACCTT |