| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168201 |
| Chromosome: | chromosome 14 |
| Location: | 4091790 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633900 | (1 of 1) K01008 - selenide, water dikinase (selD, SEPHS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCTGCATACCGTAGTAATTGCTCCCAT |
| Internal bar code: | TGGCGAAGGCGAGTGTCATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 87.5065 |
| LEAP-Seq n confirming: | 26910 |
| LEAP-Seq n nonconfirming: | 3842 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTGGAATGTTCGGTGCT |
| Suggested primer 2: | TGAGAAAAGTGGAGGTCGCT |