| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168202 |
| Chromosome: | chromosome 9 |
| Location: | 506814 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404100 | CLV3 | Voltage-gated chloride channel; (1 of 2) PTHR11689//PTHR11689:SF92 - CHLORIDE CHANNEL // H(+)/CL(-) EXCHANGE TRANSPORTER 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGCGGGGTTGGAACGAGCCGGGGGGG |
| Internal bar code: | GTACATGCACGAGAGGGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 97.0455 |
| LEAP-Seq n confirming: | 3219 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTCGTGGTGATGTTGAG |
| Suggested primer 2: | GCTGGAGAAACAGCCTCAAC |