Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.168278 |
Chromosome: | chromosome_3 |
Location: | 8135807 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g200700 | FAP166 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTACGTTGATGGGGACGATGCGGTAGTGGC |
Internal bar code: | GGTGACCATTGAGGGAAAATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1030 |
LEAP-Seq percent confirming: | 92.9651 |
LEAP-Seq n confirming: | 7810 |
LEAP-Seq n nonconfirming: | 591 |
LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAAGGCTACGAGGTCACTC |
Suggested primer 2: | TGACGCCTTTGTGTCTATGC |