| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168298 |
| Chromosome: | chromosome 10 |
| Location: | 4842774 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGAGGTGGTCAGGCAGCAGGTCAATG |
| Internal bar code: | CTCCACAGCACCTGTGGAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 99.4152 |
| LEAP-Seq n confirming: | 170 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCACCGACATTGTCCAAC |
| Suggested primer 2: | GCGTTGTCGGCCTTATACAT |