| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168299 |
| Chromosome: | chromosome 2 |
| Location: | 7406670 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144600 | PTB9 | Sodium/phosphate symporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGACCCACGACTGTTTCCATCTCGCG |
| Internal bar code: | AATTGACACCACATAAAGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1057 |
| LEAP-Seq percent confirming: | 99.4526 |
| LEAP-Seq n confirming: | 4542 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTATGTTCCCGCACTTGG |
| Suggested primer 2: | ACGTTCCAAAAGCGGTACAC |