| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168337 |
| Chromosome: | chromosome 2 |
| Location: | 4760763 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102551 | CSV1 | Chlamydomonas-specific family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGCCCAAGGCCCCAGGATCCAAGCAAG |
| Internal bar code: | GAGTGAAACCGGTTGTGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 49.9179 |
| LEAP-Seq n confirming: | 608 |
| LEAP-Seq n nonconfirming: | 610 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTACATGCTGTGGCCT |
| Suggested primer 2: | GAGACCTGGGGTCCTCCTAC |