| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168337 |
| Chromosome: | chromosome 2 |
| Location: | 4760797 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102551 | CSV1 | Chlamydomonas-specific family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGTCCTGCAGTAGGGCGGGTGGCCCGT |
| Internal bar code: | TTCCGAGAGTCTCTGGTCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 99.5255 |
| LEAP-Seq n confirming: | 2307 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTACATGCTGTGGCCT |
| Suggested primer 2: | GAGACCTGGGGTCCTCCTAC |