| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168348 |
| Chromosome: | chromosome 6 |
| Location: | 2315898 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267250 | MKP2 | (1 of 3) K04459 - dual specificity MAP kinase phosphatase (DUSP, MKP); Dual-specificity protein phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCTCGCGGTGTCTCACGCAGCCGCGCG |
| Internal bar code: | GCGTCGTGTCTATGTGGGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 99.4867 |
| LEAP-Seq n confirming: | 2326 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGGGACGAGACTGAG |
| Suggested primer 2: | CTTCAGCATCAGCACACTGC |