| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168352 |
| Chromosome: | chromosome 12 |
| Location: | 5098272 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526750 | RBM8A,RBM8 | RNA binding motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAATGGCAGGGCAACTCGCATGGAGCAC |
| Internal bar code: | ACGACGCGTGTCGTCGTATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 98.6722 |
| LEAP-Seq n confirming: | 4533 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCAGCGTAGCTGACGTA |
| Suggested primer 2: | ATGTGTTTGGTGCTGTTGGA |