Insertion junction: LMJ.RY0402.168356_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.168356
Chromosome:chromosome 6
Location:3512491
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278107 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGTTAATCCATCCACCGCTCGCGACTAC
Internal bar code:TCTAGTGGACGCTGCCGGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:410
LEAP-Seq percent confirming:99.8129
LEAP-Seq n confirming:1600
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATAGAAGCGTTCGGGAC
Suggested primer 2:AGGTGAGTCCATTTGTTGGC