| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168359 |
| Chromosome: | chromosome 6 |
| Location: | 3485621 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278099 | (1 of 2) IPR003382 - Flavoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCATCCCACGCAAAGGCAACATTCAAT |
| Internal bar code: | TGATCTATTCAACCCGGCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 97.5207 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGCATGTTTGGACTGA |
| Suggested primer 2: | CCTACCTTCTGGACAGCGAG |