| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168365 |
| Chromosome: | chromosome 13 |
| Location: | 3311010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586450 | NAT31 | N-acetyltransferase; (1 of 5) PF13508 - Acetyltransferase (GNAT) domain (Acetyltransf_7) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTATGTATCTGTACACTGAACACATAGA |
| Internal bar code: | CAAGGGGGGGTGGCAGTCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1054 |
| LEAP-Seq percent confirming: | 94.5748 |
| LEAP-Seq n confirming: | 1290 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAAGACCTTGCAGACGGC |
| Suggested primer 2: | ACGGAAGGGGAGAAGAGGTA |