| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168385 |
| Chromosome: | chromosome 7 |
| Location: | 4731731 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345200 | (1 of 1) 3.6.1.11 - Exopolyphosphatase / Metaphosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTAGTACGGTGCTAGTACGACGGCCCGG |
| Internal bar code: | AAAGGCAGGTCGTGAGGTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 82.1053 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGACCGCGAAAATGTTAC |
| Suggested primer 2: | GACTGACAGCAAATCACGGA |