| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168396 |
| Chromosome: | chromosome 2 |
| Location: | 7888214 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141550 | (1 of 2) 3.4.21.53 - Endopeptidase La / ATP-dependent serine proteinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGATTGATGGGCGTGGAGAGGGCAGAC |
| Internal bar code: | GTCCAGTCCGAAAGTAAAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 99.8105 |
| LEAP-Seq n confirming: | 4214 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCGCAATGTACTCGCAC |
| Suggested primer 2: | GCCCCACCTGTACTTGAAAA |