| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168409 |
| Chromosome: | chromosome 5 |
| Location: | 1956537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241636 | (1 of 1) PTHR23042:SF72 - TRANSCRIPTION FACTOR BHLH104-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGTCCGCAATCGGGATCCTTCCTGTAG |
| Internal bar code: | GTGTTGTGTTGATAATTGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 99.8196 |
| LEAP-Seq n confirming: | 2213 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGCGTCACTCAAGTTGT |
| Suggested primer 2: | TATTCCCCGTCTCTGTTTGC |