| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168433 |
| Chromosome: | chromosome 3 |
| Location: | 4865189 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179300 | SRH6 | (1 of 1) K11367 - chromodomain-helicase-DNA-binding protein 1 [EC:3.6.4.12] (CHD1); SNF2-related DNA/RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGGCGCAGGCATGTTGATCCGAGCGTA |
| Internal bar code: | ATACTGGTTTAAAATATTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 99.8464 |
| LEAP-Seq n confirming: | 650 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTTACCACTGTCCCAT |
| Suggested primer 2: | CATACATGATTTTTGGGGGC |