| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168439 |
| Chromosome: | chromosome 1 |
| Location: | 4212825 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028000 | POB12 | Proteome of basal body 12; (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAGGGGGCTGACGAGGACTGAGACCAA |
| Internal bar code: | GTGTGCTAACGTTCTCCGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 98.7952 |
| LEAP-Seq n confirming: | 164 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGAGCTGGCTCTGGTGCT |
| Suggested primer 2: | CGTGTGAGTGCCAAGAGTGT |