| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168440 |
| Chromosome: | chromosome 5 |
| Location: | 1951913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241636 | (1 of 1) PTHR23042:SF72 - TRANSCRIPTION FACTOR BHLH104-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCACGCCACGCACCCTCCTCCTGCCGC |
| Internal bar code: | GGATGGCACGCTCAGCTGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.3139 |
| LEAP-Seq n confirming: | 4053 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGGCAAACAAAATGTAAA |
| Suggested primer 2: | CGGTGCTTCAGGGATATGTT |