| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168445 |
| Chromosome: | chromosome 5 |
| Location: | 778418 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248300 | NRAMP4 | Manganese/metal transporter, NRAMP homolog; (1 of 1) K12347 - natural resistance-associated macrophage protein (SLC11A, NRAMP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTAATAAATGCCAGTGTTGGTTGGCGC |
| Internal bar code: | GCACGATAAGATGCACGATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.3846 |
| LEAP-Seq n confirming: | 323 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTAGGGCACGGTTGTAGG |
| Suggested primer 2: | TTCCGTCCTATCTCCTCCCT |