| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168450 |
| Chromosome: | chromosome 13 |
| Location: | 406141 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564550 | RPC17 | DNA-directed RNA polymerase III subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACCGACCGATAGTCGACTACCGTATTA |
| Internal bar code: | GCGACTGTCTCTGCAGAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 99.7274 |
| LEAP-Seq n confirming: | 2927 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTACCTTTCTTCGCAGCC |
| Suggested primer 2: | CCATTCCAGCCAACTGTTTT |