| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168476 |
| Chromosome: | chromosome 8 |
| Location: | 4389041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381900 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTCCTCACGATGTAGCTAACAGCTGAG |
| Internal bar code: | GCGGTCCGACTTGTATGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 99.397 |
| LEAP-Seq n confirming: | 989 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTCCGAGTTGCCATTAT |
| Suggested primer 2: | GTGGGTGAAGGTTAAGAGCG |